CRISPR-Cas toolbox for basic and applied genetics

Kutubuddin Molla

** ICAR-National Rice Research Institute, Cuttack-753006, India.

kutubuddin.molla@icar.gov.in

Abstract

The development of CRISPR/Cas genome editing system provided us unprecedented ability to manipulate a target genome. Day by day, we are witnessing an explosion of CRISPR/Cas-derived tools for basic and applied research. This rapid advancement has been possible by exploiting the natural diversity of the CRISPR system coupled with protein engineering. The CRISPR-Cas toolbox has too many to offer, including but not limited to conventional targeted gene knockout, gene activation and repression, epigenome editing, single-base alteration, and precise insertion/deletion/replacement with either HDR or prime editing. My talk is intended to present an overview of the spectrum of CRISPR-Cas-derived tools and their mode of action and potential application. 

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Keywords: CRISPR/Cas9, Orthologous Cas proteins, Base editing, Prime editing, genetic manipulation