Genome editing in wheat

Hikmet Budak .

** Montana BioAg. Inc. Missoula, MT, USA.

hikmet.budak@icloud.com

Abstract

Genome editing (CRISPR) can be used as precision breeding to (i) introduce genetics diversity and (ii) develop new wheat varieties with enhanced yield and end-use quality. It can also be used as a powerful tool for knockout generation, precise modification, multiplex modification, and the activation and repression of target gene(s). In this presentation, we will talk about two RNA- guided genome editing for genetic diversity and different CRISPR proteins such as Cpf1 (CRISPR from Prevotella and Francisella 1) in place of the more commonly used Cas9 for common genes. A study of editing cadmium genes in durum and bread wheats using multiplex modification will also will be outlined and further discussed.

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Keywords: CRISPR-Cas9; CRISPR-Cpf1; Gene editing; Wheat.

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